A549 Cell Line Origin:
The A549 cell line was first developed in 1972 b D.J Giard, et al. through the removal and culturing of cancerous lung tissue in the explanted tumor of 58-year-old caucausian male.
A549 Cytogenetic Information:
The cell line is hypotriploid with a modal chromosome number of 66, which occurs in 24% of cells. modal number 64 and 67 is relatively common with higher ploidies occurring at an infrequent rate (0.4%)
Morphology and Characteristics of A549 Cells:
A549 cells are human alveolar basal epithelial cells. These are squamous in nature and responsible for the diffusion of substances, such as water and electrolytes, across the alveoli of lungs. They grow adherently, as a monolayer, in vivo.
The squamous epithelial cells are positive for keratin, as is evidenced by immunoperoxidase staining. These cells are also able to synthesize lecithin and contain a high percentage of desaturated fatty acids, which are utilized by the cytidine-diphospho-choline pathway and important for the maintenance of membrane phospholipids in cells.